- Title
- Effects of gender, cytokine gene polymorphisms and environmental factors on inflammatory responses
- Creator
- Moscovis, Sophia M.; Cox, Amanda; Hall, Sharron T.; Burns, Christine J.; Scott, Rodney J.; Blackwell, C. Caroline
- Relation
- Innate Immunity Vol. 21, Issue 5, p. 523-530
- Publisher Link
- http://dx.doi.org/10.1177/1753425914553645
- Publisher
- Sage
- Resource Type
- journal article
- Date
- 2015
- Description
- Previous studies have indicated that cytokine gene polymorphisms of Indigenous Australians were predominantly associated with strong pro-inflammatory responses. We tested the hypothesis that cells of donors with genetic profiles of inflammatory cytokine single nucleotide polymorphisms (SNPs) similar to Indigenous Australians produce higher pro-inflammatory responses. PBMCs from 14 donors with genetic profiles for a high risk of strong pro-inflammatory responses and 14 with low-risk profiles were stimulated with endotoxin and effects of gender, IFN-γ, cigarette smoke extract (CSE) and testosterone on cytokine responses analysed. Cytokines were calculated from standard curves (Luminex 2.3 software). No significant differences were associated with SNP profile alone. Lower pro-inflammatory responses were observed for cells from males with low- or high-risk profiles. For cells from females with high-risk profiles, anti-inflammatory IL-10 responses were significantly reduced. There was no effect of testosterone levels on responses from males. For females, results from IFN-γ-treated cells showed positive correlations between testosterone levels and IL-1β responses to endotoxin for both risk groups and TNF-α for the high-risk group. If interactions observed among CSE, IFN-γ, genetic background and testosterone reflect those in vivo, these might contribute to increased incidences of hospitalisations for infectious diseases among Indigenous women.
- Subject
- cytokines; gene polymorphisms; cigarette smoke; gender; Indigenous Australians; endotoxin; interferon-γ
- Identifier
- http://hdl.handle.net/1959.13/1332984
- Identifier
- uon:26990
- Identifier
- ISSN:1753-4259
- Language
- eng
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