- Title
- The effect of Schizandrol A-induced DNA methylation on SH-SY5YAB 1-40 altered neuronal cell line: a potential use in Alzheimers disease
- Creator
- Zhang, Ming; Huo, Dong-sheng; Cai, Zhi-ping; Shao, Guo; Wang, He; Zhao, Zhi-ying; Yang, Zhan-jun
- Relation
- Journal of Toxicology and Environmental Health. Part A: Current Issues Vol. 78, Issue 21-22, p. 1321-1327
- Publisher Link
- http://dx.doi.org/10.1080/15287394.2015.1085942
- Publisher
- Taylor & Francis
- Resource Type
- journal article
- Date
- 2015
- Description
- Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimers disease (AD). The SH-SY5Y neuronal cell line treated with Aβ1-40 (5 μmol/L) protein is considered to be a model of AD. Hence the aim of this study was to examine the influence of Schizandrol A, a plant extract, on DNA methylation in SH-SY5Y cells exposed to Aβ1-40. Aβ1-40 were incubated with varying concentrations of Sehizandrol A to a final concentration of 1 (low), 3 (intermediate) or 9 μg/ml (high). Exposure of SH-SY5Y with Aβ1-40 reduced viability, and altered cellular morphology and mRNA expression of DNA methyltransferase (DNMT3A) and DNMT3B. Treatment with 1 or 3 μg/ml Sehizandrol A resulted in normal cell morphology as well as elevated cell number, enhanced viability, and increased mRNA expression of DNMT3A and DNMT3B compared to saline. However, an increase in Sehizandrol A to 9 μg/ml produced a fall in cell viability, as well as a decrease in mRNA DNMT3A and DNMT3B expression to control levels. Data demonstrated that Schizandrol A at 1 or 3 μg/ml improved cell morphological appearance and viability of Aβ1-40 injured SH-SY5Y cells by an enhanced DNA methylation pathway.
- Subject
- alzheimers disease; central nervous system; cell morphology; Schizandrol A
- Identifier
- http://hdl.handle.net/1959.13/1330541
- Identifier
- uon:26413
- Identifier
- ISSN:1528-7394
- Language
- eng
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