- Title
- Identification of TPIT and other novel autoantigens in lymphocytic hypophysitis; immunoscreening of a pituitary cDNA library and development of immunoprecipitation assays
- Creator
- Smith, Casey Jo Anne; Bensing, Sophie; Burns, Christine; Robinson, Phillip J.; Kasperlik-Zaluska, Anna A.; Scott, Rodney J.; Kampe, Olle; Crock, Patricia A.
- Relation
- Funding BodyNHMRCGrant Number100952
- Relation
- European Journal of Endocrinology Vol. 166, Issue 3, p. 391-398
- Publisher Link
- http://dx.doi.org/10.1530/EJE-11-1015
- Publisher
- Bioscientifica
- Resource Type
- journal article
- Date
- 2012
- Description
- Background: Lymphocytic hypophysitis is an organ-specific autoimmune disease of the pituitary gland. A specific and sensitive serological test currently does not exist to aid in the diagnosis. Objective: To identify target autoantigens in lymphocytic hypophysitis and develop a diagnostic assay for these proteins. Design/methods: A pituitary cDNA expression library was immunoscreened using sera from four patients with lymphocytic hypophysitis. Relevant cDNA clones from screening, along with previously identified autoantigens pituitary gland-specific factor 1a and 2 (PGSF1a and PGSF2) and neuron-specific enolase (NSE) were tested in an in vitro transcription and translation immunoprecipitation assay. The corticotroph-specific transcription factor, TPIT, was investigated separately as a candidate autoantigen. Results: Significantly positive autoantibody reactivity against TPIT was found in 9/86 hypophysitis patients vs 1/90 controls (P=0.018). The reactivity against TPIT was not specific for lymphocytic hypophysitis with autoantibodies detectable in the sera from patients with other autoimmune endocrine diseases. Autoantibodies were also detected against chromodomain-helicase-DNA binding protein 8, presynaptic cytomatrix protein (piccolo), Ca²⁺-dependent secretion activator, PGSF2 and NSE in serum samples from patients with lymphocytic hypophysitis, but at a frequency that did not differ from healthy controls. Importantly, 8/86 patients with lymphocytic hypophysitis had autoantibodies against any two autoantigens in comparison with 0/90 controls (P=0.0093). Conclusions: TPIT, a corticotroph-specific transcription factor, was identified as a target autoantigen in 10.5% of patients with lymphocytic hypophysitis. Further autoantigens related to vesicle processing were also identified as potential autoantigens with different immunoreactivity patterns in patients and controls.
- Subject
- lymphocytic hypophysitis; autoantigens; immunoscreening; TPIT
- Identifier
- http://hdl.handle.net/1959.13/1319058
- Identifier
- uon:23772
- Identifier
- ISSN:0804-4643
- Rights
- © 2012 European Society of Endocrinology. This is an Open Access article distributed under the terms of the European Journal of Endocrinology's Re-use Licence which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
- Language
- eng
- Full Text
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