- Title
- Investigation of gene expression underpinning partitioning of seed storage compunds in legumes
- Creator
- Song, Youhong; Liao, Siyang; Wang, Xin-Ding; Sheahan, Michael B.; Garg, Manohar L.; Rose, Ray J.
- Relation
- 16th Agronomy Conference 2012. "Capturing Opportunities and Overcoming Obstacles in Australian Agronomy": Proceedings of the 16th ASA Conference (Armidale, NSW 14-18 October, 2012)
- Relation
- http://www.regional.org.au/au/asa/2012/breeding/7896_songyh.htm
- Publisher
- Australian Society of Agronomy
- Resource Type
- conference paper
- Date
- 2012
- Description
- Legume seed store considerable protein and oil, and also starch in some species, however, the composition of storage compounds largely varies with the species. The molecular mechanism of seed storage partitioning remains to be clarified. In this paper, Medicago truncatula (the model legume) and Medicago orbicularis were used to compare the molecular basis underlying the partitioning of seed storage compounds. Seeds of M. orbicularis and M. truncatula were sampled at 10, 14, 18, 22 and 26 days after anthesis and at the time of maturation. M. orbicularis had a similar dry weight (4 mg per seed) to M. truncatula, but only accumulated around a third of seed storage protein and oil. The less seed protein and oil content was due to the lower expression of master transcription factors LEAFY COTYLEDON1-Like (L1L) and ABA INSENSITIVE 3 (ABI3), in M. orbicularis. M. orbicualris had similar expression of AGPase (a major enzyme controlling starch synthesis) to M. truncatula, suggesting that M. orbicularis did not switch carbon flow to the starch pathway. The findings in this study can be implied for molecular breeding or genetic transformation towards improving protein/oil production for food nutrition or biofuel purposes.
- Subject
- gene regulation; transcription factors; seed; protein and oil storage
- Identifier
- http://hdl.handle.net/1959.13/1313397
- Identifier
- uon:22578
- Language
- eng
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