- Title
- Imprinted DLK1-DIO3 region of 14q32 defines a schizophrenia-associated miRNA signature in peripheral blood mononuclear cells
- Creator
- Gardiner, E.; Beveridge, N. J.; Wu, J. Q.; Carr, V.; Scott, R. J.; Tooney, P. A.; Cairns, M. J.
- Relation
- NHMRC.631057
- Relation
- Molecular Psychiatry Vol. 17, p. 827-840
- Publisher Link
- http://dx.doi.org/10.1038/mp.2011.78
- Publisher
- Nature Publishing Group
- Resource Type
- journal article
- Date
- 2012
- Description
- MicroRNAs (miRNAs) regulate gene expression at the post-transcriptional level and are important for coordinating nervous system development and neuronal function in the mature brain. We have recently identified schizophrenia-associated alteration of cortical miRNA biogenesis and expression in post-mortem brain tissue with implications for the dysregulation of schizophrenia candidate genes. Although these changes were observed in the central nervous system, it is plausible that schizophrenia-associated miRNA expression signatures may also be detected in non-neural tissue. To explore this possibility, we investigated the miRNA expression profile of peripheral blood mononuclear cells (PBMCs) from 112 patients with schizophrenia and 76 non-psychiatric controls. miRNA expression analysis of total RNA conducted using commercial miRNA arrays revealed that 33 miRNAs were significantly downregulated after correction for multiple testing with a false discovery rate (FDR) of 0%, which increased to 83 when we considered miRNA with an FDR<5%. Seven miRNAs altered in microarray analysis of schizophrenia were also confirmed to be downregulated by quantitative real-time reverse transcription-polymerase chain reaction. A large subgroup consisting of 17 downregulated miRNAs is transcribed from a single imprinted locus at the maternally expressed DLK1-DIO3 region on chromosome 14q32. This pattern of differentially expressed miRNA in PBMCs may be indicative of significant underlying genetic or epigenetic alteration associated with schizophrenia
- Subject
- biomarker; gene silencing; microRNA; PBMCs; schizophrenia; 14q32
- Identifier
- http://hdl.handle.net/1959.13/1296986
- Identifier
- uon:19349
- Identifier
- ISSN:1359-4184
- Language
- eng
- Full Text
- Reviewed
- Hits: 13901
- Visitors: 6740
- Downloads: 651
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT02 | Publisher version (open access) | 633 KB | Adobe Acrobat PDF | View Details Download |