- Title
- A simple, rapid, and reliable protocol to localize hydrogen peroxide in large plant organs by DAB-mediated tissue printing
- Creator
- Liu, Yong-Hua; Offler, Christina E.; Ruan, Yong-Ling
- Relation
- Frontiers in Plant Science Vol. 5, p. 1-6
- Publisher Link
- http://dx.doi.org/10.3389/fpls.2014.00745
- Publisher
- Frontiers Research Foundation
- Resource Type
- journal article
- Date
- 2014
- Description
- Hydrogen peroxide (H₂O₂) is a major reactive oxygen species (ROS) and plays diverse roles in plant development and stress responses. However, its localization in large and thick plant organs (e.g., stem, roots, and fruits), other than leaves, has proven to be challenging due to the difficulties for the commonly used H₂O₂-specific chemicals, such as 3,3'-diaminobenzidine (DAB), cerium chloride (CeCl₃), and 2',7'-dichlorofluorescin diacetate (H₂DCF-DA), to penetrate those organs. Theoretically, the reaction of endogenous H₂O₂ with these chemicals could be facilitated by using thin organ sections. However, the rapid production of wound-induced H₂O₂ associated with this procedure inevitably disturbs the original distribution of H₂O₂ in vivo. Here, by employing tomato seedling stems and fruits as testing materials, we report a novel, simple, and rapid protocol to localize H₂O₂ in those organs using DAB-mediated tissue printing. The rapidity of the protocol (within 15 s) completely avoided the interference of wound-induced H₂O₂ during experimentation. Moreover, the H₂O₂ signal on the printing was stable for at least 1 h with no or little background produced. We conclude that DAB-mediated tissue printing developed here provide a new feasible and reliable method to localize H₂O₂ in large plant organs, hence should have broad applications in studying ROS biology.
- Subject
- fruits and stems; hydrogen peroxide; localization; tissue printing; reactive oxygen species
- Identifier
- http://hdl.handle.net/1959.13/1293861
- Identifier
- uon:18693
- Identifier
- ISSN:1664-462X
- Language
- eng
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