- Title
- miR-10b promotes migration and invasion in nasopharyngeal carcinoma cells
- Creator
- Sun, Xiao-Jin; Liu, Hao; Zhang, Pei; Zhang, Xu-Dong; Jiang, Zhi-Wen; Jiang, Chen Chen
- Relation
- Asian Pacific Journal of Cancer Prevention Vol. 14, Issue 9, p. 5533-5537
- Publisher Link
- http://dx.doi.org/10.7314/APJCP.2013.14.9.5533
- Publisher
- Asian Pacific Organization for Cancer Prevention
- Resource Type
- journal article
- Date
- 2013
- Description
- MicroRNA-10b (miR-10b) has been reported to play an important role in some types of cancer, but the effects and possible mechanisms of action of miR-10b in the metastasis of nasopharyngeal carcinoma cells (NPC) have not been explored. The aim of the present study was to investigate the function of miR-10b in nasopharyngeal carcinoma and to determine the molecular mechanisms underlying its action. The MTT assay was used to assess proliferation of CNE-2Z cells. Wound healing and transwell migration assays were applied to assess cell migration and invasion, while and expression of E-cadherin and MMP-9 were detected using Western blot analysis. Real-time PCR was employed to detect the expression of genes related to migration and invasion and the 2-ΔΔCt method was used to calculate the degree of expression. MTT assay showed the expression of miR-10b to have no effect on the proliferation of NPC cell lines. The wound healing assay showed that miR-10b mimics promoted the mobility and invasion of NPC cell lines. Inhibitors of miR-10b reduced the ability of NPC cell lines to migrate and invade. In addition, the expression of genes related to migration and invasion, such as E-cadherin, vimentin, and MMP-9, were confirmed to be different in the CNE-2Z NPC cell line transfected with miR-10b mimics and with miR-10b inhibitors. In the present study, miR-10b was found to upregulate the expression of MMP-9 and knockdown of miR-10b was found to significantly downregulate the expression of E-cadherin. On the whole, these results showed that miR-10b plays an important role in the invasion and metastasis of NPC cells.
- Subject
- miR-10b; NPC; invasion; metastasis
- Identifier
- http://hdl.handle.net/1959.13/1044666
- Identifier
- uon:14359
- Identifier
- ISSN:1513-7368
- Language
- eng
- Full Text
- Reviewed
- Hits: 1152
- Visitors: 1622
- Downloads: 242
| Thumbnail | File | Description | Size | Format | |||
|---|---|---|---|---|---|---|---|
| View Details Download | ATTACHMENT01 | Publisher version (open access) | 755 KB | Adobe Acrobat PDF | View Details Download |