- Title
- Plant regeneration: somatic embryogenesis
- Creator
- Nolan, Kim E.; Rose, Ray J.
- Relation
- Plant Cell Culture: Essential Methods p. 39-59
- Publisher Link
- http://dx.doi.org/10.1002/9780470686522.ch3
- Publisher
- Wiley-Blackwell Publishing
- Resource Type
- book chapter
- Date
- 2010
- Description
- In somatic embryogenesis (SE), embryos form asexually from somatic cells. SE is most commonly associated with the in vitro culture of excised tissues in a nutrient medium containing exogenously supplied plant growth regulators. However, SE can occur naturally as on the succulent leaves of Kalanchoe, and a type of SE can also occur naturally in vivo through the process of apomixis. Plants which undergo apomixis develop embryos in the ovule without fertilization and fertile seed is produced with the same genotype as the parent. The methods in this chapter are concerned with SE in vitro and the use of the term 'SE' will be in the context of the in vitro form. SE is used in transformation procedures for many species.
- Subject
- plant regeneration; somatic embryogenesis; embryos; in vitro cultures
- Identifier
- http://hdl.handle.net/1959.13/932739
- Identifier
- uon:11438
- Identifier
- ISBN:9780470686485
- Language
- eng
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