https://ogma.newcastle.edu.au/vital/access/manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:52394 Wed 28 Feb 2024 15:33:56 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:10324 Wed 24 Jul 2013 22:27:14 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:10323 Wed 24 Jul 2013 22:27:13 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:48597 Wed 22 Mar 2023 08:46:48 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:53475 Wed 21 Feb 2024 14:57:04 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:36606 Wed 17 Nov 2021 16:31:32 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:35848 streptomyces species. Despite the significant increase in the antibacterial resistant pathogens, aminoglycosides remain an important class of antimicrobial drugs due to their unique chemical structure which offers a broad spectrum of activity. The modification of antibiotics and their subsequent use in supramolecular chemistry is rarely reported. Given the importance of aminoglycosides, here we give a brief overview on the modification of 4,5- and 4,6-disubstituted deoxystreptamine classes of aminoglycosides through supramolecular chemistry and their potential for real world applications. We also make the case that the work in this area is gaining momentum, and there are significant opportunities to meet the challenges of modern antibiotics through the modification of aminoglycosides by harnessing the advantages of supramolecular chemistry.]]> Wed 11 Dec 2019 12:45:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:29783 dif. In Escherichia coli, two tyrosine-family recombinases, XerC and XerD, bind to dif and carry out two pairs of sequential strand exchange reactions. However, what makes the reaction unique among site-specific recombination reactions is that the first step, XerD-mediated strand exchange, relies on interaction with the very C-terminus of the FtsK DNA translocase. FtsK is a powerful molecular motor that functions in cell division, co-ordinating division with clearing chromosomal DNA from the site of septation and also acts to position the dif sites for recombination. This is a model system for unlinking, separating and segregating large DNA molecules. Here we describe the molecular detail of the interaction between XerD and FtsK that leads to activation of recombination as deduced from a co-crystal structure, biochemical and in vivo experiments. FtsKγ interacts with the C-terminal domain of XerD, above a cleft where XerC is thought to bind. We present a model for activation of recombination based on structural data.]]> Wed 11 Apr 2018 17:12:14 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:12573 Wed 11 Apr 2018 15:46:41 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:3261 Wed 11 Apr 2018 14:44:37 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:7836 Wed 11 Apr 2018 12:59:01 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:7727 Wed 11 Apr 2018 10:46:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:4380 Wed 11 Apr 2018 10:34:48 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:15863 Wed 11 Apr 2018 10:29:57 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:9705 Wed 11 Apr 2018 09:51:44 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:18830 Wed 11 Apr 2018 09:26:28 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:48119 Wed 06 Mar 2024 14:55:07 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:29924 Wed 04 Sep 2019 12:16:26 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:39718 270,000 independent mQTLs, of which 8.5% comprise long-range (trans) associations. Identified mQTL associations explain 15-17% of the additive genetic variance of DNAm. We show that the genetic architecture of DNAm levels is highly polygenic. Using shared genetic control between distal DNAm sites, we constructed networks, identifying 405 discrete genomic communities enriched for genomic annotations and complex traits. Shared genetic variants are associated with both DNAm levels and complex diseases, but only in a minority of cases do these associations reflect causal relationships from DNAm to trait or vice versa, indicating a more complex genotype-phenotype map than previously anticipated.]]> Tue 21 Mar 2023 17:20:57 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:32666 capacitation in order to attain the competence to recognize the egg and then engage in a complex cascade of cell–cell interactions in order to achieve union of the gametes at fertilization. This process involves extensive remodelling of the sperm plasma membrane as well as the induction of hyperactivated motility and, as such, is a highly energy-dependent process. The process of spermatogenesis requires extensive remodelling of a conventional spherical cell to become one of the most highly specialized and morphologically differentiated cells in the body. During this transformation, the DNA in the sperm nucleus reaches the physical limits of compaction to achieve a quasicrystalline state. This extreme compaction requires the removal or resorption of most of the cytoplasm, at the same time removing the majority of the organelles (such as the endoplasmic reticulum, ribosomes and Golgi apparatus) that are intimately involved in the regulation of metabolism in somatic cells. The result of this extensive remodelling is that spermatozoa are left translationally and transcriptionally silent, as well as relatively depleted of intracellular enzymes and energy reserves such as fat droplets, yolk granules and glycogen. For this reason, spermatozoa are heavily dependent on their immediate extracellular environment for the energy substrates that drive metabolism, as well as a variety of specialized enzymatic activities that would normally be conducted intracellularly. For example, in somatic cells, the array of enzymes and low-molecular-mass scavengers involved in mediating protection against oxidative stress is housed intracellularly, largely within the cytoplasmic space. Spermatozoa, on the other hand, largely depend upon the epididymal and seminal plasmas to provide the richest and most diverse combination of antioxidants in the body, including several that are unique to the male reproductive tract. In much the same way that economies trade using a currency rather than a barter system, biological systems have all evolved their own unique ‘currencies’ for the exchange of energy.The most important of these currencies is adenosine 5’-triphosphate (ATP), which provides the metabolic energy to drive activities in all living cells.]]> Tue 10 Jul 2018 11:47:12 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:35827 Tue 10 Dec 2019 11:54:23 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:37758 regenerating islet-derived (REG) gene family are important regulators of many cellular processes. Here we functionally characterise a non-protein coding product of the family, the long noncoding RNA (lncRNA) REG1CP that is transcribed from a DNA fragment at the family locus previously thought to be a pseudogene. REG1CP forms an RNA–DNA triplex with a homopurine stretch at the distal promoter of the REG3A gene, through which the DNA helicase FANCJ is tethered to the core promoter of REG3A where it unwinds double stranded DNA and facilitates a permissive state for glucocorticoid receptor α (GRα)-mediated REG3A transcription. As such, REG1CP promotes cancer cell proliferation and tumorigenicity and its upregulation is associated with poor outcome of patients. REG1CP is also transcriptionally inducible by GRα, indicative of feedforward regulation. These results reveal the function and regulation of REG1CP and suggest that REG1CP may constitute a target for cancer treatment.]]> Thu 27 Jan 2022 15:55:02 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:7491 Sat 24 Mar 2018 08:37:45 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:9647 Sat 24 Mar 2018 08:35:25 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:14315 Sat 24 Mar 2018 08:24:40 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:10662 Sat 24 Mar 2018 08:12:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:10322 Sat 24 Mar 2018 08:07:01 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:17995 Sat 24 Mar 2018 07:56:35 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:6762 Sat 24 Mar 2018 07:47:15 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:5320 Sat 24 Mar 2018 07:45:58 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:5642 Sat 24 Mar 2018 07:44:01 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:396 Sat 24 Mar 2018 07:42:32 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:28373 Escherichia coli sliding clamp occurs via a sequential mechanism that involves two subsites (I and II). Here, we report the development of small-molecule inhibitors that mimic this mechanism. The compounds contain tetrahydrocarbazole moieties as "anchors" to occupy subsite I. Functional groups appended at the tetrahydrocarbazole nitrogen bind to a channel gated by the side chain of M362 and lie at the edge of subsite II. One derivative induced the formation of a new binding pocket, termed subsite III, by rearrangement of a loop adjacent to subsite I. Discovery of the extended binding area will guide further inhibitor development.]]> Sat 24 Mar 2018 07:35:58 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:27570 Sat 24 Mar 2018 07:23:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:50787 Sat 05 Aug 2023 11:22:45 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:33931 Mon 23 Sep 2019 13:28:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:54758 Mon 11 Mar 2024 15:01:25 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:19570 Mon 10 Aug 2015 12:20:19 AEST ]]> https://ogma.newcastle.edu.au/vital/access/manager/Repository/uon:13309 20TP a novel code sequence is comprised of 158 DNA bases derived from an ostensibly non-coding region of a gene. This gene known as the APOE4 gene (Apolipoprotein E4) is associated with Alzheimer’s disease in humans. This sequence was inserted into E.coli bacteria and used as temporal paint-media to depict small living portraits. Through this process a previously inanimate, non-coding section of DNA is poetically expressed in interplay of memories and learning within a simulated 1970s childhood classroom.]]> Fri 01 Sep 2017 16:11:31 AEST ]]>