Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes | NOVA. The University of Newcastle's Digital Repository

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.13/804239

Title: Essential CDK1-inhibitory role for separase during meiosis I in vertebrate oocytes
Author/Creator: Gorr, Ingo H.; Reis, Alexandra; Boos, Dominik; Wühr, Martin; Madgwick, Suzanne; Jones, Keith T.; Stemmann, Olaf
Description: Separase not only triggers anaphase of meiosis I by proteolytic cleavage of cohesin on chromosome arms, but in vitro vertebrate separase also acts as a direct inhibitor of cyclin-dependent kinase 1 (Cdk1) on liberation from the inhibitory protein, securin. Blocking separase–Cdk1 complex formation by microinjection of anti-separase antibodies prevents polar-body extrusion in vertebrate oocytes. Importantly, proper meiotic maturation is rescued by chemical inhibition of Cdk1 or expression of Cdk1-binding separase fragments lacking cohesin-cleaving activity.
Relation: Nature Cell Biology Vol. 8, Issue 9, p. 1035-1037
Publisher Link: http://dx.doi.org/10.1038/ncb1467
Date: 2006
Publisher: Nature Publishing Group
Keyword(s): cdk1; meiosis; oocytes; anaphase; proteolytic; chromosome
Resource Type: journal article
Identifier: http://hdl.handle.net/1959.13/804239
Identifier: ISSN:1465-7392
Language: eng

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