Of the three intracellular Ca²⁺ signalling molecules (InsP₃, cADPR and NAADP)sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and NAADP. Homogenate is prepared in a Na⁺/Cl⁻ substitute of N-Methyl glucamine (NMG)/gluconate. To determine how media previous termcompositionnext term affects Ca²⁺ release we replaced NMG with various sugars or glycine and found a dramatic improvement in InsP₃ mediated Ca²⁺ release. Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media previous termcompositionnext term may be an important consideration in using homogenate to study Ca²⁺ release for future studies.
Biochemical and Biophysical Research Communications Vol. 360, Issue 4, p. 815-820